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Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910144101803321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910829884203321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910840664503321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
The hypercycle : a principle of natural self-organization / M. Eigen, P. Schuster
| The hypercycle : a principle of natural self-organization / M. Eigen, P. Schuster |
| Autore | Eigen, M. |
| Pubbl/distr/stampa | Berlin : Springer-Verlag, 1979 |
| Descrizione fisica | viii, 92 p. : ill. ; 27 cm. |
| Altri autori (Persone) | Schuster, P.author |
| Soggetto topico | Genetic translation |
| ISBN | 0387092935 |
| Classificazione |
53.1.65
574 QH371.E33 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991000999259707536 |
Eigen, M.
|
||
| Berlin : Springer-Verlag, 1979 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas
| Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas |
| Autore | Liljas Anders |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 |
| Descrizione fisica | 1 online resource (339 p.) |
| Disciplina |
572.645
572/.633 |
| Soggetto topico |
Proteins - Synthesis
Ribosomes - Structure Genetic translation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-87716-6
9786611877163 981-256-578-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Preface; Contents; List of Abbreviations; 1 The Basics of Translation; 2 Historical Milestones; 3 Methods to Study Structure; 4 The Message - mRNA; 5 The Adaptor - tRNA; 6 The Workbench - Ribosomes; 7 The Structure of the Ribosome; 8 Ribosomal Sites and Ribosomal States; Color Plates; 9 The Catalysts - Translation Factors; 10 Inhibitors of Protein Synthesis, Antibiotics, Resistance; 11 The Process - Translation; 12 Protein Folding and Targeting; 13 Evolution of the Translation Apparatus; Appendix I; Appendix II; References; Index |
| Record Nr. | UNINA-9910450194603321 |
|
Liljas Anders
|
||
| Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas
| Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas |
| Autore | Liljas Anders |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 |
| Descrizione fisica | 1 online resource (339 p.) |
| Disciplina |
572.645
572/.633 |
| Soggetto topico |
Proteins - Synthesis
Ribosomes - Structure Genetic translation |
| ISBN |
1-281-87716-6
9786611877163 981-256-578-7 |
| Classificazione | 35.75 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Preface; Contents; List of Abbreviations; 1 The Basics of Translation; 2 Historical Milestones; 3 Methods to Study Structure; 4 The Message - mRNA; 5 The Adaptor - tRNA; 6 The Workbench - Ribosomes; 7 The Structure of the Ribosome; 8 Ribosomal Sites and Ribosomal States; Color Plates; 9 The Catalysts - Translation Factors; 10 Inhibitors of Protein Synthesis, Antibiotics, Resistance; 11 The Process - Translation; 12 Protein Folding and Targeting; 13 Evolution of the Translation Apparatus; Appendix I; Appendix II; References; Index |
| Record Nr. | UNINA-9910783640003321 |
|
Liljas Anders
|
||
| Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas
| Structural aspects of protein synthesis [[electronic resource] /] / Anders Liljas |
| Autore | Liljas Anders |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 |
| Descrizione fisica | 1 online resource (339 p.) |
| Disciplina |
572.645
572/.633 |
| Soggetto topico |
Proteins - Synthesis
Ribosomes - Structure Genetic translation |
| ISBN |
1-281-87716-6
9786611877163 981-256-578-7 |
| Classificazione | 35.75 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Preface; Contents; List of Abbreviations; 1 The Basics of Translation; 2 Historical Milestones; 3 Methods to Study Structure; 4 The Message - mRNA; 5 The Adaptor - tRNA; 6 The Workbench - Ribosomes; 7 The Structure of the Ribosome; 8 Ribosomal Sites and Ribosomal States; Color Plates; 9 The Catalysts - Translation Factors; 10 Inhibitors of Protein Synthesis, Antibiotics, Resistance; 11 The Process - Translation; 12 Protein Folding and Targeting; 13 Evolution of the Translation Apparatus; Appendix I; Appendix II; References; Index |
| Record Nr. | UNINA-9910820960003321 |
|
Liljas Anders
|
||
| Singapore ; ; Hackensack, N.J., : World Scientific Pub. Co., c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Translation
| Translation |
| Pubbl/distr/stampa | Austin, Texas : , : Landes Bioscience, , [2013]- |
| Descrizione fisica | 1 online resource |
| Disciplina | 572.88 |
| Soggetto topico |
Messenger RNA
Genetic translation RNA, Messenger Translational Research, Biomedical |
| Soggetto genere / forma |
Periodical
Fulltext Internet Resources. Periodicals. |
| ISSN | 2169-0731 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-996205883403316 |
| Austin, Texas : , : Landes Bioscience, , [2013]- | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Translation
| Translation |
| Pubbl/distr/stampa | Austin, Texas : , : Landes Bioscience, , [2013]- |
| Descrizione fisica | 1 online resource |
| Disciplina | 572.88 |
| Soggetto topico |
Messenger RNA
Genetic translation RNA, Messenger Translational Research, Biomedical |
| Soggetto genere / forma |
Periodical
Fulltext Internet Resources. Periodicals. |
| ISSN | 2169-0731 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910139066903321 |
| Austin, Texas : , : Landes Bioscience, , [2013]- | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Translational control of gene expression [[electronic resource] /] / edited by Nahum Sonenberg, John W.B. Hershey, Michael B. Mathews
| Translational control of gene expression [[electronic resource] /] / edited by Nahum Sonenberg, John W.B. Hershey, Michael B. Mathews |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Cold Spring Harbor, NY, : Cold Spring Harbor Laboratory Press, c2000 |
| Descrizione fisica | x, 1020 p. : ill. (some col.) |
| Altri autori (Persone) |
SonenbergNahum
HersheyJohn W. B MathewsMichael |
| Collana | Cold Spring Harbor monograph series |
| Soggetto topico | Genetic translation |
| ISBN | 0-87969-676-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910780169403321 |
| Cold Spring Harbor, NY, : Cold Spring Harbor Laboratory Press, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
